Enrichment and cultivation of microorganisms from sediment from the slope of the Peru Trench (ODP Site 1230)

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doi: 10.2973/odp.proc.sr.201.107.2005
Author(s): Biddle, Jennifer F.; House, Christopher H.; Brenchley, Jean E.
Ocean Drilling Program, Leg 201, Shipboard Scientific Party, College Station, TX
Author Affiliation(s): Primary:
Pennsylvania State University, Department of Geosciences, University Park, PA, United States
Other:
University of Rhode Island, United States
Ocean Drilling Program, United States
University of California Santa Cruz, United States
U. S. Geological Survey, United States
Yale University, United States
University of Bristol, United Kingdom
Carl von Ossietzky Universität Oldenburg, Federal Republic of Germany
Rice University, United States
New Mexico Institute of Mining and Technology, United States
Lamont-Doherty Earth Observatory, United States
Woods Hole Oceanographic Institution, United States
Stockholm University, Sweden
Pennsylvania State University, United States
Japan Marine Science and Technology Center, Japan
ETH Zurich, Switzerland
University of Texas at Dallas, United States
Texas A&M University, United States
Shinshu University, Japan
Federal Institute for Geosciences and Natural Resources, Federal Republic of Germany
Sydney University of Technology, Australia
University of Georgia, United States
Volume Title: Proceedings of the Ocean Drilling Program; scientific results; controls on microbial communities in deeply buried sediments, eastern Equatorial Pacific and Peru Margin; covering Leg 201 of the cruises of the drilling vessel JOIDES Resolution; San Diego, California, to Valparaiso, Chile; Sites 1225-1231; 27 January-29 March 2002
Volume Author(s): Jorgensen, Bo B., editor; D'Hondt, Steven L.; Miller, D. Jay; Aiello, Ivano W.; Bekins, Barbara; Blake, Ruth E.; Cragg, Barry A.; Cypionka, Heribert; Dickens, Gerald R.; Ferdelman, Timothy G.; Ford, Kathryn H.; Gettemy, Glen L.; Guèrin, Gilles; Hinrichs, Kai-Uwe; Holm, Nils G.; House, Christopher H.; Inagaki, Fumio; Meister, Patrick; Mitterer, Richard M.; Naehr, Thomas H.; Niitsuma, Sachiko; Parkes, R. John; Schippers, Axel; Skilbeck, C. Gregory; Smith, David C.; Spivack, Arthur J.; Teske, Andreas P.; Wiegel, Juergen
Source: Proceedings of the Ocean Drilling Program; scientific results; controls on microbial communities in deeply buried sediments, eastern Equatorial Pacific and Peru Margin; covering Leg 201 of the cruises of the drilling vessel JOIDES Resolution; San Diego, California, to Valparaiso, Chile; Sites 1225-1231; 27 January-29 March 2002, Bo B. Jorgensen, Steven L. D'Hondt, D. Jay Miller, Ivano W. Aiello, Barbara Bekins, Ruth E. Blake, Barry A. Cragg, Heribert Cypionka, Gerald R. Dickens, Timothy G. Ferdelman, Kathryn H. Ford, Glen L. Gettemy, Gilles Guèrin, Kai-Uwe Hinrichs, Nils G. Holm, Christopher H. House, Fumio Inagaki, Patrick Meister, Richard M. Mitterer, Thomas H. Naehr, Sachiko Niitsuma, R. John Parkes, Axel Schippers, C. Gregory Skilbeck, David C. Smith, Arthur J. Spivack, Andreas P. Teske and Juergen Wiegel; Ocean Drilling Program, Leg 201, Shipboard Scientific Party, College Station, TX. Proceedings of the Ocean Drilling Program, Scientific Results (CD ROM), Vol.201, 19p. Publisher: Texas A&M University, Ocean Drilling Program, College Station, TX, United States. ISSN: 1096-2514
Note: In English. Available only on CD-ROM in PDF format and on the Web in PDF or HTML. 32 refs.CD-ROM, ISSN 1096-2514; WWW, ISSN 1096-7451; illus., incl. 2 tables
Summary: The deep biosphere is estimated to hold a significant percentage of the Earth's prokaryotic biomass; however, little is known about the organisms in this environment. Here, we describe investigations of the diversity of microorganisms enriched from surface and subsurface sediment collected during Leg 201 of the Ocean Drilling Program at Site 1230 on the slope of the Peru Trench. This site contains methane hydrates, high levels of organic matter, and high direct cell counts, all of which indicate the potential for thriving microbial populations. To investigate these populations, we examined prokaryotes in samples from seafloor to 258 meters below seafloor (mbsf) using both cultivation and molecular methods. From seafloor samples, we cultivated isolates representing the genera Photobacterium, Shewanella, and Halomonas. The population found in an enrichment cultivated at low temperatures, 0.67 mbsf, contained many cell morphologies and deoxyribonucleic acid (DNA) signatures, but this population, except for a Vibrio sp., was difficult to separate and grow as pure cultures. Most isolates produced extracellular lytic enzymes that were active at low temperatures. Methanogens have been expected to play a large role in the creation of methane hydrates in the sediment; therefore, we also attempted to enrich for psychrophilic methanogens. No methane was found above background levels in anaerobic enrichments incubated for 2 yr, nor was any 16S ribosomal DNA detected following amplification using archaeal primers with DNA extracted from these incubated cultures. These results illustrate the need for further extensive microbiological studies in order to understand the biogeochemistry of this important subseafloor environment.
Year of Publication: 2006
Research Program: ODP Ocean Drilling Program
Key Words: 07 Marine Geology and Oceanography; Biochemistry; Biomass; Continental margin; Continental slope; Cores; DNA; Deep-sea environment; East Pacific; Ecology; Equatorial Pacific; Experimental studies; Geochemistry; Leg 201; Living taxa; Marine environment; Marine sediments; Microorganisms; ODP Site 1230; Ocean Drilling Program; Pacific Ocean; Peru; Peru-Chile Trench; Phylogeny; Prokaryotes; Sediments; South America
Coordinates: S090700 S090700 W0803500 W0803500
Record ID: 2007008191
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