Microbial diversity in sediment near Grizzly Bare Seamount in holes U1363B and U1363G

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doi: 10.2204/iodp.proc.327.201.2013
Author(s): Jungbluth, Sean P.; Johnson, Lani G. H.; Cowen, James P.; Rappe, Michael S.
Integrated Ocean Drilling Program, Expedition 327 Scientists, College Station, TX
Author Affiliation(s): Primary:
University of Hawaii at Manoa, Hawaii Institute of Marine Biology, Honolulu, HI, United States
Other:
Kyoto University, Japan
Texas A&M University, United States
Lamont-Doherty Earth Observatory, United States
University of Miami, United States
University of Hawaii at Manoa, United States
University of Southampton, United Kingdom
Tongji University, China
École Normale Supérieure, France
Arhus Universitet, Denmark
University of Southern California, United States
University of Alaska at Fairbanks, United States
Volume Title: Proceedings of the Integrated Ocean Drilling Program; Juan de Fuca Ridge-flank hydrology; Expedition 327 of the riserless drilling platform from and to Victoria, British Columbia (Canada); Sites U1362, U1301, 1027, and U1363, 5 July-5 September 2010
Volume Author(s): Fisher, Andrew; Tsuji, Takeshi; Petronotis, Katerina; Mrozewski, Stefan; Becker, Keir; Cowen, James P.; Harris, Michelle; Hulme, Samuel M.; Inderbitzen, Katherine; Ji Fuwu; Masui, Reona; Miyamoto, Hiroki; Morvan, Sylvain; Orcutt, Beth; Rutter, Jennifer; Turner, Amanda G.; Wheat, C. Geoffrey; Winslow, Dustin M.
Source: Proceedings of the Integrated Ocean Drilling Program; Juan de Fuca Ridge-flank hydrology; Expedition 327 of the riserless drilling platform from and to Victoria, British Columbia (Canada); Sites U1362, U1301, 1027, and U1363, 5 July-5 September 2010, Andrew Fisher, Takeshi Tsuji, Katerina Petronotis, Stefan Mrozewski, Keir Becker, James P. Cowen, Michelle Harris, Samuel M. Hulme, Katherine Inderbitzen, Ji Fuwu, Reona Masui, Hiroki Miyamoto, Sylvain Morvan, Beth Orcutt, Jennifer Rutter, Amanda G. Turner, C. Geoffrey Wheat and Dustin M. Winslow; Integrated Ocean Drilling Program, Expedition 327 Scientists, College Station, TX. Proceedings of the Integrated Ocean Drilling Program (Online), Vol.327, 27p. Publisher: IODP Management International, Washington, DC, United States. ISSN: 1930-1014
Note: In English. 68 refs.; illus., incl. sect., 3 tables, sketch map
Summary: Microorganisms inhabiting sediment in close proximity to recharging basement outcrops are of interest because of the enhanced advective fluid flow in these locations, which is expected to exert unique selective pressures on the resident microbial communities. Here, Integrated Ocean Drilling Program (IODP) boreholes were used to access sediment microbial communities near Grizzly Bare recharge seamount on the Juan de Fuca Ridge eastern flank. The two locations examined in this study, Holes U1363G and U1363B, are 50 and 177 m away from the center of the outcrop, respectively. In general, small subunit ribosomal RNA gene clones from all three domains of life were detected; these groups were predominantly related to microorganisms known to reside in marine sediment. A large fraction of environmental gene clones recovered from Hole U1363B and U1363G sediment were related to uncultivated, candidate phyla of Bacteria such as BHI80-139, BRC1, JS1, OPB41, and TA06. Hole U1363B and U1363G sediment clone libraries were generally dominated by the domain Bacteria and particularly the phylum Chloroflexi, which comprised approximately one-quarter of the total gene clones identified. However, borehole sediment also contained several archaeal lineages that were phylogenetically affiliated with the Miscellanenous Crenarchaeotal Group. Eukaryotic fungi were only detected within the interstitial water sample from Hole U1363B. Finally, a minor portion of clones recovered from sediment in this study were also recovered from basement fluid samples previously characterized from Baby Bare discharge seamount and Ocean Drilling Program and IODP borehole Circulation Obviation Retrofit Kit Observatories (CORKs) in Holes 1026B and U1301A, which are ∼50 km to the north-northeast.
Year of Publication: 2013
Research Program: IODP Integrated Ocean Drilling Program
Key Words: 07 Marine Geology and Oceanography; Archaea; Bacteria; Biogenic processes; Boreholes; Communities; Cores; East Pacific; Expedition 327; Genetics; Grizzly Bare Seamount; Hydrothermal vents; IODP Site U1363; Integrated Ocean Drilling Program; Juan de Fuca Ridge; Marine sediments; Microorganisms; North Pacific; Northeast Pacific; Nucleic acids; Pacific Ocean; Phylogeny; RNA; Sediments
Coordinates: N471719 N471736 W1280145 W1280210
Record ID: 2013101150
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