Cell Alive System (CAS); a new method of core sample freezing for shore-based biological analyses and sample storage

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doi: 10.1180/minmag.2013.077.5.24
Author(s): Xiao, Nan; Morono, Yuki; Terada, Takeshi; Yamamoto, Yuhji; Horise, Takehiro; Inagaki, Fumio
Author Affiliation(s): Primary:
Japan Agency for Marine-Earth Science and Technology, Kochi Institute for Core Sample Research, Kochi, Japan
Other:
Marine Works Japan, Japan
Kochi University, Japan
Volume Title: Goldschmidt abstracts 2013
Source: Mineralogical Magazine, 77(5), p.2521; Goldschmidt 2013, Florence, Italy, Aug. 25-30, 2013. Publisher: Mineralogical Society, London, United Kingdom. ISSN: 0026-461X
Note: In English
Summary: We report a novel freezing technology for the long-term preservation of subseafloor core samples. Subseafloor core samples recovered by scientific ocean drilling provide unprecedented opportunities to study deep subseafloor life and biogeocheminal cycles. For the future analyses of cores using newly developed life science technologies, archiving precious core materials under the appropriate condition is fundamentary significant. Given such scientific requirements, the Kochi Core Center (KCC), one of the official core repositories of the Integrated Ocean Drilling Program (IODP), has started storing some biological core samples in -80°C deep freezers and/or in liquid N2 tanks, so called "DeepBIOS" (Deep Biosphere Samples). To keep quality assurance and control (QA/QC) of the DeepBIOS, the initital freezing process is a key: however, using the conventional way (e.g., quick transfer to deep freezer), it has been confirmed that formation of ice crystals decompose biological signatures. During the JAMSTEC Chikyu Expedition 905, we tested a new technology called "Cell Alive System (CAS)", which utilizes magnetic field to vibrate water molecule in the sample, following snap and hence uniform freezing of core samples at the supercooling temperature. The core samples from various depths were sub-sampled, and immediately frozen in the CAS system along with the standard freezing method under the temperature of -20°C, -80°C, and -196°C. Analysis of cell abundance showed that conventional freezing methods decreased the number of microbial cells, whereas the CAS freezing resulted in almost no loss of the cells. We also tested the paleomagnetic characteristics after the CAS freezing, indicating no or very little change in remnant magnetism. No visible changes in volume of sediment was observed after the CAS freezing. Consequently, our results indicate that the CAS freezing technique is highly useful for QA/QC of scientific frozen core samples to preserve intact biological signatures, as well as other non-biological characteristics, for the long-term storage.
Year of Publication: 2013
Research Program: IODP Integrated Ocean Drilling Program
Key Words: 07 Marine Geology and Oceanography; Cell Alive System; Cores; Freezing; Integrated Ocean Drilling Program; Kochi Core Center; Marine sediments; Preservation; Samples; Sediments; Technology
Record ID: 2014052598
Copyright Information: GeoRef, Copyright 2019 American Geosciences Institute. Abstract, Copyright, Mineralogical Society of Great Britain and Ireland

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