Drilling fluid contamination during riser drilling quantified by chemical and molecular tracers

Author(s): Inagaki, F.; Lever, M. A.; Morono, Y.; Hoshino, T.
Author Affiliation(s): Primary:
Japan Agency for Marine-Earth Science and Technology, Kochi Institute for Core Sample Research, Nankoku, Japan
Other:
Aarhus University, Center for Geomicrobiology, Aarhus, Denmark
Volume Title: AGU 2012 fall meeting
Source: American Geophysical Union Fall Meeting, Vol.2012; American Geophysical Union 2012 fall meeting, San Francisco, CA, Dec. 3-7, 2012. Publisher: American Geophysical Union, Washington, DC, United States
Note: In English
Summary: Stringent contamination controls are essential to any type of microbiological investigation, and are particularly challenging in ocean drilling, where samples are retrieved from hundreds of meters below the seafloor. In summer 2012, Integrated Ocean Drilling Expedition 337 aboard the Japanese vessel Chikyu pioneered the use of chemical tracers in riser drilling while exploring the microbial ecosystem of coalbeds 2 km below the seafloor off Shimokita, Japan. Contamination tests involving a perfluorocarbon tracer that had been successfully used during past riserless drilling expeditions were complemented by DNA-based contamination tests. In the latter, likely microbial contaminants were targeted via quantitative polymerase chain reaction assays using newly designed, group-specific primers. Target groups included potential indicators of (a) drilling mud viscosifiers (Xanthomonas, Halomonas), (b) anthropogenic wastewater (Bifidobacterium, Blautia, Methanobrevibacter), and (c) surface seawater (SAR 11, Marine Group I Archaea). These target groups were selected based on past evidence suggesting viscosifiers, wastewater, and seawater as the main sources of microbial contamination in cores retrieved by ocean drilling. Analyses of chemical and molecular tracers are in good agreement, and indicate microorganisms associated with mud viscosifiers as the main contaminants during riser drilling. These same molecular analyses are then extended to subseafloor samples obtained during riserless drilling operations. General strategies to further reduce the risk of microbial contamination during riser and riserless drilling operations are discussed.
Year of Publication: 2012
Research Program: IODP Integrated Ocean Drilling Program
Key Words: 30 Engineering Geology; Drilling; Drilling muds; Expedition 337; Integrated Ocean Drilling Program; North Pacific; Northwest Pacific; Pacific Ocean; West Pacific
Coordinates: N411035 N411036 E1421202 E1421201
Record ID: 2014092650
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