Fungal diversity from deep marine subsurface sediments (IODP 317, Canterbury Basin, New Zealand)

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Author(s): Redou, V.; Arzur, D.; Burgaud, G.; Barbier, G.
Author Affiliation(s): Primary:
Université Européenne de Bretagne, Plouzane, France
Volume Title: AGU 2012 fall meeting
Source: American Geophysical Union Fall Meeting, Vol.2012; American Geophysical Union 2012 fall meeting, San Francisco, CA, Dec. 3-7, 2012. Publisher: American Geophysical Union, Washington, DC, United States
Note: In English
Summary: Recent years have seen a growing interest regarding micro-eukaryotic communities in extreme environments as a third microbial domain after Bacteria and Archaea. However, knowledge is still scarce and the diversity of micro-eukaryotes in such environments remains hidden and their ecological role unknown. Our research program is based on the deep sedimentary layers of the Canterbury Basin in New Zealand (IODP 317) from the subsurface to the record depth of 1884 meters below seafloor. The objectives of our study are (i) to assess the genetic diversity of fungi in deep-sea sediments and (ii) identify the functional part in order to better understand the origin and the ecological role of fungal communities in this extreme ecosystem. Fingerprinting-based methods using capillary electrophoresis single-strand conformation polymorphism and denaturing high-performance liquid chromatography were used as a first step to raise our objectives. Molecular fungal diversity was assessed using amplification of ITS1 (Internal Transcribed Spacer 1) as a biomarker on 11 samples sediments from 3.76 to 1884 meters below seafloor. Fungal molecular signatures were detected throughout the sediment core. The phyla Ascomycota and Basidiomycota were revealed with DNA as well as cDNA. Most of the phylotypes are affiliated to environmental sequences and some to common fungal cultured species. The discovery of a present and metabolically active fungal component in this unique ecosystem allows some interesting first hypotheses that will be further combined to culture-based methods and deeper molecular methods (454 pyrosequencing) to highlight essential information regarding physiology and ecological role of fungal communities in deep marine sediments.
Year of Publication: 2012
Research Program: IODP Integrated Ocean Drilling Program
Key Words: 07 Marine Geology and Oceanography; Biodiversity; Canterbury Basin; Cores; Ecology; Ecosystems; Expedition 317; Fungi; Integrated Ocean Drilling Program; Marine sediments; Pacific Ocean; Sediments; South Pacific; Southwest Pacific; West Pacific
Coordinates: S445700 S444600 E1720200 E1714000
Record ID: 2014092704
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