Data report; Cultivation of microorganisms from basaltic rock and sediment cores from the North Pond on the western flank of the Mid-Atlantic Ridge, IODP Expedition 336

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doi: 10.2204/iodp.proc.336.204.2015
Author(s): Hirayama, Hisako; Abe, Mariko; Miyazaki, Junichi; Sakai, Sanae; Nagano, Yuriko; Takai, Ken
Integrated Ocean Drilling Program, Expedition 336 Scientists, College Station, TX
Author Affiliation(s): Primary:
Japan Agency for Marine-Earth Science and Technology, Department of Subsurface Geobiological Analysis and Research, Yokosuka, Japan
Other:
University of Bremen, Germany
Texas A&M University, United States
University of Leicester, United Kingdom
Université des Sciences et Technologies de Lille 1, France
University of Miami, United States
U. S. Geological Survey, United States
Geological Survey of Japan, Japan
Japan Agency for Marine-Earth Science and Technology, Japan
Moss Landing Marine Laboratories, United States
Universitetet i Bergen, Norway
University of Rhode Island, United States
Institut de Physique du Globe de Paris, France
Aarhus University, Denmark
Korea Institute of Geoscience and Mineral Resources, South Korea
University of Cambridge, United Kingdom
Institut Français de Recherche pour l'Exploitation de la Mer, France
University of Delaware, United States
Osaka University, Japan
Jet Propulsion Laboratory, United States
Shanghai Jiao Tong University, China
University of Alaska, Fairbanks, United States
Volume Title: Proceedings of the Integrated Ocean Drilling Program; Mid-Atlantic Ridge microbiology; initiation of long-term coupled microbiological, geochemical, and hydrological experimentation within the seafloor at North Pond, western flank of the Mid-Atlantic Ridge; Expedition 336 of the riserless drilling platform Bridgetown, Barbados, to Ponta Delgada, Azores (Portugal); Sites 395 and U1382-U1384, 16 September-16 November 2011
Volume Author(s): Edwards, Katrina J.; Bach, Wolfgang; Klaus, Adam; Anderson, Louise; Backert, Nicolas; Becker, Keir; Griffin, Dale W.; Haddad, Amanda G.; Harigane, Yumiko; Hirayama, Hisako; Hulme, Samuel M.; Jorgensen, Steffen Leth; Insua, Tania Lado; Le Campion, Paul; Mills, Heath J.; Nakamura, Kentaro; Orcutt, Beth N.; Park, Young-Soo; Rennie, Victoria; Rouxel, Olivier; Russel, Joseph A.; Sakata, Kasumi; Salas, Everett C.; Wang Fengping; Wheat, C. Geoffrey
Source: Proceedings of the Integrated Ocean Drilling Program; Mid-Atlantic Ridge microbiology; initiation of long-term coupled microbiological, geochemical, and hydrological experimentation within the seafloor at North Pond, western flank of the Mid-Atlantic Ridge; Expedition 336 of the riserless drilling platform Bridgetown, Barbados, to Ponta Delgada, Azores (Portugal); Sites 395 and U1382-U1384, 16 September-16 November 2011, Katrina J. Edwards, Wolfgang Bach, Adam Klaus, Louise Anderson, Nicolas Backert, Keir Becker, Dale W. Griffin, Amanda G. Haddad, Yumiko Harigane, Hisako Hirayama, Samuel M. Hulme, Steffen Leth Jorgensen, Tania Lado Insua, Paul Le Campion, Heath J. Mills, Kentaro Nakamura, Beth N. Orcutt, Young-Soo Park, Victoria Rennie, Olivier Rouxel, Joseph A. Russel, Kasumi Sakata, Everett C. Salas, Wang Fengping and C. Geoffrey Wheat; Integrated Ocean Drilling Program, Expedition 336 Scientists, College Station, TX. Proceedings of the Integrated Ocean Drilling Program. Expedition Reports, Vol.336, 12p. Publisher: International Ocean Discovery Program, Washington, DC, United States. ISSN: 1930-1014 CODEN: IDSDA6
Note: In English. 39 refs.; illus., incl. 4 tables
Summary: Cultivation experiments targeting chemolithoautotrophic microorganisms were performed using subseafloor basaltic cores (the deepest sample is from 315 meters below seafloor [mbsf] and overlying sediment cores (the deepest sample is from 91.4 mbsf) from North Pond on the western flank of the Mid-Atlantic Ridge. The cores were recovered by the R/V JOIDES Resolution during Integrated Ocean Drilling Program Expedition 336. Different bacteria were grown under different media and temperature conditions. In the enrichment cultures of the basaltic cores under aerobic conditions, frequently detected bacteria at 8°C and 25°C were members of the genera Ralstonia (the class Betaproteobacteria) and Pseudomonas (Gammaproteobacteria), whereas members of the genera Paenibacillus (Bacilli) and Acidovorax (Betaproteobacteria) were conspicuous at 37°C. Bacillus spp. (Bacilli) were outstanding at 37°C under anaerobic conditions. In the enriched cultures of the sediment cores, bacterial growth was observed at 15°C but not at 37°C, and the bacteria detected at 15°C mostly belonged to gammaproteobacterial genera such as Pseudomonas , Halomonas , and Marinobacter . All of the bacteria detected in this study were enriched only, and subcultivation of the enriched cultures in the respective original media did not succeed. The presence of hydrogenotrophic methanogens was examined by a culture-dependent or a culture-independent analysis in the basalt and sediment cores but was not proven. A fungal isolate was obtained from a single basaltic core an d belonged to the genus Exophiala of the order Chaetothyriales.
Year of Publication: 2015
Research Program: IODP Integrated Ocean Drilling Program
ODP Ocean Drilling Program
Key Words: 07 Marine Geology and Oceanography; Aliphatic hydrocarbons; Alkanes; Archaea; Atlantic Ocean; Bacteria; Basalts; Biochemistry; Biota; Boreholes; Cores; Crust; DNA; Expedition 336; Experimental studies; Fungi; Geomicrobiology; Hydrocarbons; IODP Site U1382; IODP Site U1383; IODP Site U1384; Igneous rocks; Integrated Ocean Drilling Program; Laboratory studies; Living taxa; Marine sediments; Methane; Microorganisms; Mid-Atlantic Ridge; North Atlantic; Nucleic acids; Ocean Drilling Program; Oceanic crust; Organic compounds; RNA; Sediments; Volcanic rocks
Coordinates: N224521 N224521 W0460453 W0460453
N224808 N224808 W0460310 W0460310
N224843 N224843 W0460521 W0460521
Record ID: 2015058078
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