Isolation and characterization of gram-positive piezophilic bacteria from deep marine subsurface sediment

Author(s): Runko, G. M.; Fang, J.; Kato, C.
Author Affiliation(s): Primary:
Hawaii Pacific University, Kaneohe, HI, United States
Other:
Japan Agency for Marine-Earth Science and Technology, Japan
Volume Title: AGU 2014 fall meeting
Source: American Geophysical Union Fall Meeting, Vol.2014; American Geophysical Union 2014 fall meeting, San Francisco, CA, Dec. 15-19, 2014. Publisher: American Geophysical Union, Washington, DC, United States
Note: In English
Summary: The marine deep biosphere remains as the least studied of all of Earth's habitats and is inadequately understood, but is extremely important to understand the impacts that microbes have on global biogeochemical cycles. Sediment samples were obtained during IODP Expedition 337 in the western Pacific Ocean, from 1,498 meters below the seafloor (mbsf; samples 6R3), 1,951-1,999 mbsf (19R1), and 2,406 mbsf (29R7). These samples were initially mixed with marine broth and cultivated under anaerobic conditions at pressure of 35 MPa (megapascal) and temperatures of 35°C, 45°C, and 55°C for 3 months on board the Chikyu. Single colonies were isolated via plating on marine broth. Then, six strains of bacteria were identified, 6R3-1, 6R3-15, 19R1-5, 29R7-12B, 29R7-12M, and 29R7-12S. The six strains were then examined for optimal growth temperature and pressure. These organisms are Gram-positive, spore-forming, facultative anaerobic piezophilic bacteria. Major fatty acids are anteiso-15:0, anteiso-17:0 and iso-15:0. Phylogenetic analysis of 16S rRNA gene sequences revealed that the isolates are closely related to Virgibacillus pantothenticus, Robinsoniella peoriensis, and Bacillus subtilis. Because of their abundance in the deep marine subsurface, these microorganisms likely play an important role in sustaining the deep microbial ecosystem and influencing biogeochemical cycles in the deep biosphere.
Year of Publication: 2014
Research Program: IODP Integrated Ocean Drilling Program
Key Words: 22 Environmental Geology; Bacteria; Expedition 337; Integrated Ocean Drilling Program; Marine sediments; North Pacific; Northwest Pacific; Nucleic acids; Pacific Ocean; RNA; Sediments; West Pacific
Coordinates: N411035 N411036 E1421202 E1421201
Record ID: 2015092472
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