Deep sequencing of subseafloor eukaryotic rRNA reveals active fungi across marine subsurface provinces

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doi: 10.1371/journal.pone.0056335
Author(s): Orsi, William; Biddle, Jennifer F.; Edgcomb, Virginia
Author Affiliation(s): Primary:
Woods Hole Oceanographic Institution, Department of Geology and Geophysics, Wood Hole, MA, United States
University of Delaware, United States
Volume Title: PLoS One
Source: PLoS One, 2013(e56335), p.1-12. Publisher: Public Library of Science, San Francisco, CA, United States. ISSN: 1932-6203
Note: In English. 61 refs.; illus., incl. 2 tables
Summary: The deep marine subsurface is a vast habitat for microbial life where cells may live on geologic timescales. Because DNA in sediments may be preserved on long timescales, ribosomal RNA (rRNA) is suggested to be a proxy for the active fraction of a microbial community in the subsurface. During an investigation of eukaryotic 18S rRNA by amplicon pyrosequencing, unique profiles of Fungi were found across a range of marine subsurface provinces including ridge flanks, continental margins, and abyssal plains. Subseafloor fungal populations exhibit statistically significant correlations with total organic carbon (TOC), nitrate, sulfide, and dissolved inorganic carbon (DIC). These correlations are supported by terminal restriction length polymorphism (TRFLP) analyses of fungal rRNA. Geochemical correlations with fungal pyrosequencing and TRFLP data from this geographically broad sample set suggests environmental selection of active Fungi in the marine subsurface. Within the same dataset, ancient rRNA signatures were recovered from plants and diatoms in marine sediments ranging from 0.03 to 2.7 million years old, suggesting that rRNA from some eukaryotic taxa may be much more stable than previously considered in the marine subsurface.
Year of Publication: 2013
Research Program: ODP Ocean Drilling Program
Key Words: 09 Paleontology, Paleobotany; Algae; Cenozoic; Chemical composition; Correspondence analysis; Diatoms; East Pacific; Equatorial Pacific; Eukaryotes; Floral studies; Fungi; Hydrate Ridge; Leg 201; Leg 204; Marine environment; Marine sediments; Microfossils; Neogene; North Pacific; Northeast Pacific; Nucleic acids; ODP Site 1225; ODP Site 1227; ODP Site 1244; Ocean Drilling Program; Pacific Ocean; Paleoenvironment; Plantae; Pleistocene; Pliocene; Preservation; Quaternary; RNA; Sediments; South Pacific; Southeast Pacific; Statistical analysis; Tertiary; Upper Pliocene
Coordinates: N443500 N443500 W1250700 W1250700
S090000 S090000 W0795700 W0795700
N024600 N024600 W1103400 W1103400
Record ID: 2016076933
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