Archaeoglobus sulfaticallidus sp. nov., a thermophilic and facultatively lithoautotrophic sulfate-reducer isolated from black rust exposed to hot ridge flank crustal fluids

Online Access: Get full text
doi: 10.1099/ijs.0.016105-0
Author(s): Steinsbu, Bjorn O.; Thorseth, Ingunn H.; Nakagawa, Satoshi; Inagaki, Fumio; Lever, Mark A.; Engelen, Bert; Ovreas, Lise; Pedersen, Rolf B.
Author Affiliation(s): Primary:
University of Bergen, Centre for Geobiology, Bergen, Norway
Other:
Japan Agency for Marine-Earth Science and Technology, Japan
University of North Carolina at Chapel Hill, United States
Universität Oldenburg, Germany
Volume Title: International Journal of Systematic and Evolutionary Microbiology
Source: International Journal of Systematic and Evolutionary Microbiology, 60(12), p.2745-2752. Publisher: Society for General Microbiology, Reading, United Kingdom. ISSN: 1466-5026
Note: In English. 44 refs.; illus., incl. 1 table
Summary: A novel thermophilic and lithoautotrophic sulfate-reducing archaeon was isolated from black rust formed on the steel surface of a borehole observatory (CORK 1026B) retrieved during IODP Expedition 301 on the eastern flank of Juan de Fuca Ridge, eastern Pacific Ocean. Cells of the strain were lobe-shaped or triangular. The optimum temperature, pH and NaCl concentration for growth were 75 6C, pH 7 and 2 % (w/v), respectively. The isolate was strictly anaerobic, growing lithoautotrophically on H2 and CO2 using sulfate, sulfite or thiosulfate as electron acceptors. Lactate and pyruvate could serve as alternative energy and carbon sources. The G+C content of the genomic DNA was 42 mol%. Phylogenetic analyses of the 16S rRNA gene indicated that the isolate was closely related to members of the family Archaeoglobaceae, with sequence similarities of 90.3-94.4 %. Physiological and molecular properties showed that the isolate represents a novel species of the genus Archaeoglobus. The name Archaeoglobus sulfaticallidus sp. nov. is proposed; the type strain is PM70-1T (5DSM 19444T 5JCM 14716T ).
Year of Publication: 2010
Research Program: IODP Integrated Ocean Drilling Program
ODP Ocean Drilling Program
Key Words: 07 Marine Geology and Oceanography; Archaea; Archaeoglobus sulfaticallidus; DNA; East Pacific; Expedition 301; Growth; Integrated Ocean Drilling Program; Juan de Fuca Ridge; Microorganisms; New taxa; North Pacific; Northeast Pacific; Nucleic acids; ODP Site 1026; Ocean Drilling Program; Pacific Ocean; RNA; Reduction; Sulfates
Coordinates: N474556 N474556 W1274526 W1274526
Record ID: 2016083760
Copyright Information: GeoRef, Copyright 2019 American Geosciences Institute.