Oceanobacillus pacificus sp. nov., isolated from a deep-sea sediment

Online Access: Get full text
doi: 10.1099/ijs.0.056481-0
Author(s): Yu Cong; Yu Shaolan; Zhang Zenghu; Li Zhao; Zhang Xiao-Hua
Author Affiliation(s): Primary:
Ocean University of China, College of Marine Life Sciences, Qingdao, China
Volume Title: International Journal of Systematic and Evolutionary Microbiology
Source: International Journal of Systematic and Evolutionary Microbiology, 64(4), p.1278-1283. Publisher: Society for General Microbiology, Reading, United Kingdom. ISSN: 1466-5026
Note: In English. 37 refs.; illus., incl. table
Summary: A moderately halophilic bacterial strain, designated XH204T , was isolated from deep-sea sediment core (45° 58 S 163° 11 W) of the South Pacific Gyre during the Integrated Ocean Drilling Program Expedition 329. The strain was Gram-stain-positive, rod-shaped, motile by peritrichous flagella and produced ellipsoidal endospores subterminally positioned within swollen sporangia. Growth of strain XH204T occurred at 15-42 6C (optimum 37 6C), in the presence of 0-14 % (w/v) NaCl (optimum 4 %) and at pH 7.0-10.0 (optimum pH 8.0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XH204T belonged to the genus Oceanobacillus and showed the highest sequence similarity to Oceanobacillus profundus CLMP28T (95.6 %); strain XH204T exhibited 93.4 % 16S rRNA gene sequence similarity to the type strain of the type species of the genus Oceanobacillus, Oceanobacillus iheyensis HTE831T . The dominant fatty acids of strain XH204T were anteiso-C15:0, iso-C14:0 and iso-C16:0. The cell wall of strain XH204T contained meso-diaminopimelic acid as the diagnostic diamino acid, and ribose, glucose and galactose as the major whole-cell sugars. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. Menaquinone-7 (MK-7) was the only isoprenoid quinone and the DNA G+C content was 38.8 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic analysis, strain XH204T represents a novel species of the genus Oceanobacillus, for which the name Oceanobacillus pacificus sp. nov. is proposed. The type strain is XH204T (5DSM 25873T 5JCM 18381T ).
Year of Publication: 2014
Research Program: IODP Integrated Ocean Drilling Program
Key Words: 07 Marine Geology and Oceanography; Bacteria; Carboxylic acids; DNA; Deep-sea environment; East Pacific; Expedition 329; Fatty acids; IODP Site U1371; Integrated Ocean Drilling Program; Lipids; Living taxa; Marine environment; Marine sediments; Microorganisms; Nucleic acids; Oceanobacillus pacificus; Organic acids; Organic compounds; Pacific Ocean; RNA; Sediments; South Pacific; Southeast Pacific
Coordinates: S455751 S455751 W1631103 W1631103
Record ID: 2016083767
Copyright Information: GeoRef, Copyright 2019 American Geosciences Institute.