Diversity of actinomycetes isolated from subseafloor sediments after prolonged low-temperature storage

Online Access: Get full text
doi: 10.1007/s12223-014-0361-z
Author(s): Ulanova, Dana; Goo, Kian Sim
Author Affiliation(s): Primary:
Kochi University, Oceanography Section, Nankoku, Japan
Volume Title: Folia Microbiologica
Source: Folia Microbiologica, 60(3), p.211-216. Publisher: Springer Netherlands, Dordrecht, Netherlands. ISSN: 1874-9356
Note: In English. 36 refs.
Summary: Subseafloor sediments present an untapped source of novel bacterial species with industrially important bioactivities. Subseafloor core samples collected during the Integrated Ocean Drilling Program Expeditions 315, 316, and 331 and stored in Kochi Core Center at -80 °C for 1 to 4 years were used for cultivation-based study of viable actinomycetes. In total, more than 100 actinomycete-like colonies were isolated from two deep-frozen subseafloor sediment samples. Isolated actinomycetes showed close similarity to known Actinotalea, Dietzia, Gordonia, Isoptericola, Microbacterium, Nocardia, Rhodococcus, Pseudonocardia, Streptomyces, and Tsukamurella species and were halotolerant. Bioactivity assays revealed that two of the isolates were producing potent antibacterial compound(s) and one isolate was having antifungal activity. Our study demonstrated that deep-frozen subseafloor core samples could be a potential source of viable actinomycetes, which may be used in drug discovery.
Year of Publication: 2015
Research Program: IODP Integrated Ocean Drilling Program
Key Words: 07 Marine Geology and Oceanography; Actinomycetes; Bacteria; Communities; Expedition 315; Expedition 316; Expedition 331; Integrated Ocean Drilling Program; Marine sediments; NanTroSEIZE; North Pacific; Northwest Pacific; Okinawa Trough; Pacific Ocean; Sediments; Species diversity; West Pacific
Coordinates: N331400 N331800 E1364300 E1363800
N330100 N331400 E1364800 E1364300
N274700 N274800 E1265500 E1265300
Record ID: 2019040695
Copyright Information: GeoRef, Copyright 2019 American Geosciences Institute.